Enter the Web-Head: Listen to all of Beasts 'practical' and 'necessary' pointers
Burglary Interrupted: Shoot some B-Ball
Rooftops By Night: Defeat all 21 enemies without taking damage
Warehouse 66: Take out all enemies from the ceiling without taking damage
Spidey Vs. Shocker: Defeat The Shocker
Spidey Vs. Shocker: Defeat The Shocker with only two hits without taking damage
Smoke Screen: Collect all 4 keys before using any of them and defuse the bomb on Normal or higher difficulty
Hangar 18: Take out all enemies and machine guns without setting off your spider-sense
Wind Tunnel: Make the plane make a full circle without crashing into anything
To Catch a Thief: Catch up to the chopper in 2:00 or less
In Darkest Night: Complete the train yard puzzle using only 3 switches
Heart of Darkness: Put 10 enemies on ice
Catch That Train!: Catch up to the train and meet up with a fellow bug
Catch That Train!: Catch the train without taking damage or damaging Sandman
Gangland: Take out all the gangsters and rescue all the hostages without taking damage
Spidey Vs. Hammerhead: Defeat Hammerhead
Spidey Vs. Hammerhead: Defeat Hammerhead without taking damage
Spidey in the Machine: Complete the actuator puzzle in 5 seconds
Mission: Spidey: Complete the impossible mission without taking damage at all
The Corkscrew: Carefully make your way up the shaft without taking damage
Spidey Vs. Lizard: Defeat the Lizard and rescue your old pal, Dr. Connors
Spidey Vs. Lizard: Defeat the Lizard using only the serum without taking damage on normal or higher difficulty
The Gauntlet: Disable all 13 laser turrets with electric webbing then destroy them with melee attacks
Spidey Vs. Sandman Again: Defeat the Sandman
Spidey Vs. Sandman Again: Defeat the Sandman without taking damage, using the water no more than 5 times
Konichi-Wa, Spider-San: Destroy the generator in 3:00 or less
Rock of Ages: Scale the wall without stopping or taking damage
Spidey Vs. Electro: Defeat Electro's first form without taking damage
The Best Laid Plans: Defeat Electro's final form without taking damage, stunning him no more than 6 times
Help Thor save the world!
What if Spider-Man rode the banana?
What if Spider-Man defeated 10 enemies in warehouse 66 without webbing or melee attacks?
What if Spider-Man was good at pong?
What if Spider-Man broke the machine?
Complete the Web-Slinging Challenge in 1:10.0 or less
Complete the Web Yank Challenge in 0:25.0 or less
Complete the L1 Targeting Challenge in 0:30.0 or less
Complete the L2 Targeting Challenge with a score of 140 or better
Complete the Zip-Line Challenge in 1:30.0 or less
Complete the Stealth Challenge in 1:15.0 or less
Defeat 75 Thugs in the Attack Challenge
Defeat Electro once and for all as Spider-Phoenix
Defeat Shocker as Prodigy
Clear out Warehouse 66 as Dusk
Complete Mission: Spidey using the insulated suit
Interrupt a burglary with the Red Alex Ross suit
Catch a thief with the White Alex Ross suit
Defeat the Lizard as Venom 2 - Earth X
Wind your way up The Corkscrew in the Negative Zone suit
Defeat Electro in the Museum as Symbiote Spider-Man
Complete Spidey in the Machine as Spider-Man 2099
Defeat the Sandman as Captain Universe
Get inside Hangar 18 as Spidey Unlimited
Scale the Rock of Ages as The Amazing Bag Man!
Defeat Hammerhead as Scarlet Spidey
Catch That Train as Ben Reily
Stop the plane as Quick Change Spidey
Complete The Gauntlet as Peter Parker
Complete Enter the Web-Head as Battle-Damaged Spidey
Create an overpowered Spider-Suit
You can't say that!
Unlock all Bugle Headlines
Unlock all of Parker's Portfolio
Collect all comics
Unlock all costumes
Complete the game on Hard difficulty