Defeat Electro in Stage 1
Defeat Sandman in Stage 2
Defeat Mysterio in Stage 3
Defeat Vulture in Stage 4
Defeat Hobgoblin in Stage 5
Defeat Dr. Octopus in Stage 6 and beat the game
Beat the game without using any continues
Have at least 3 vials worth (24 units) of unused web
Get the Key to Electro's area in Stage 1
Get the TNT, rig it and blow open the barrier to Sand Man's area in Stage 2
Get the Infra-red Goggles in Stage 3
Defeat Electro in Stage 1 without taking damage
Defeat Sand Man in Stage 2 without taking damage
Defeat Mysterio in Stage 3 without taking damage
Defeat The Vulture in Stage 4 without taking damage
Defeat Hobgoblin in Stage 5 without taking damage
Defeat Dr. Octopus in Stage 6 without taking damage
Defeat Electro in Stage 1 without using any web
Defeat Sand Man in Stage 2 without using any web
Defeat Mysterio in Stage 3 without using any web
Defeat The Vulture in Stage 4 without using any web
Defeat The Hobgoblin in Stage 5 without using any web
Defeat Dr. Octopus in Stage 6 without using any web
Defeat Electro in Stage 1 with web remaining at the time of victory
Defeat Sand Man in Stage 2 with web remaining at the time of victory
Defeat Mysterio in Stage 3 with web remaining at the time of victory
Defeat The Vulture in Stage 4 with web remaining at the time of victory
Defeat The Hobgoblin in Stage 5 with web remaining at the time of victory
Defeat Dr. Octopus in Stage 6 with web remaining at the time of victory
Beat Stage 1 Area 1 without taking damage (reset game if damage is taken)
Beat Stage 1 Area 2 without taking damage (use a continue if damage is taken)
Beat Stage 2 Area 1 without taking damage (no out of bounds, use a continue if damage is taken)
Beat Stage 3 Area 1 without taking damage (use a continue if damage is taken)
Beat Stage 3 Area 2 with Goggles without taking damage (use a continue if damage is taken)
Beat Stage 3 Area 3 without taking damage (use a continue if damage is taken)
Beat Stage 3 Area 4 without taking damage (use a continue if damage is taken)
Beat Stage 4 Area 1 without taking damage (use a continue if damage is taken)
Beat Stage 5 Area 1 without taking damage (use a continue if damage is taken)
Beat Stage 6 Area 1 without taking damage (use a continue if damage is taken)
Beat the game without ever running out of web (get the web vial before killing anyone)
Beat the game without ever having or picking up web
Get a score of 10,000 points or more
Get a score of 20,000 points or more
Get a score of 30,000 points or more
Obtain all 3 web vials from the Hobgoblin's area in Stage 5