Beat the game on Normal difficulty or higher without using any test tubes

Score 100,000 points on Normal difficulty or higher

Score 200,000 points on Normal difficulty or higher

Score 300,000 points on Normal difficulty or higher

Score 400,000 points on Normal difficulty or higher

Starting with 5 or less lives, max out your lives at 9 on Normal difficulty or higher

Stockpile 6 test tubes on Normal difficulty or higher

Defeat 20 enemies using the flying attack

Defeat 20 enemies using the flying jump kick

Defeat 20 enemies using the head toss

Defeat 20 enemies using the cape sweep

Use the double whammy 20 times

Use the special throw 50 times

Defeat 20 enemies using the spear gun

Defeat 50 Bikers using Batarangs

Block 50 attacks

Hit Stun Gun Clown with a slab of concrete

Break all windows in the background of Scene 1-1

Destroy all objects in the background of Scene 2-1

Defeat 100 enemies outside of Scene 1-2

Defeat 200 enemies outside of Scene 1-2

Reach the Scene 1 boss on Normal difficulty or higher without losing any lives

Defeat Stun Gun Clown in Scene 1 on Normal difficulty or higher without losing any lives or using any test tubes

Reach the Scene 2 boss on Normal difficulty or higher without losing any lives

Defeat the Tattooed Strongman in Scene 2 on Normal difficulty or higher without losing any lives

Collect 2 Test Tubes and 2 Big Hearts in Scene 3-2

Reach the Scene 3 boss on Normal difficulty or higher without losing any lives

Defeat Catwoman in Scene 3 on Normal difficulty or higher without losing any lives

Reach Catwoman in Scene 4 on Normal difficulty or higher without losing any lives

Defeat Catwoman in Scene 4 on Normal difficulty or higher without losing any lives

Defeat The Penguin in Scene 4 on Normal difficulty or higher without losing any lives

Complete Scene 5 on Normal difficulty or higher without losing any lives

Collect 7 score items in Scene 5

Reach the Scene 6 boss on Normal difficulty or higher without losing any lives

Defeat the Organ Grinder in Scene 6 on Normal difficulty or higher without losing any lives

Reach The Penguin's Duck on Normal difficulty or higher without losing any lives

Defeat The Penguin's Duck in Scene 7 on Normal difficulty or higher without losing any lives

Defeat The Penguin in Scene 7 on Normal difficulty or higher without losing any lives

Beat the game on Mania difficulty without using any continues

Complete Scene 1 on Normal difficulty or higher

Complete Scene 2 on Normal difficulty or higher

Complete Scene 3 on Normal difficulty or higher

Complete Scene 4 on Normal difficulty or higher

Complete Scene 5 on Normal difficulty or higher

Complete Scene 6 on Normal difficulty or higher

Complete Scene 7 and beat the game on Normal difficulty or higher

Beat the game on Mania difficulty