Unlockables (and achievements for them) will not unlock if you quit a game through the pause menu. Additionally, save before entering the EEE trial as that wipes any loaded save data.
In Puzzle 99, create the full puzzle shape and destroy it, get a single-color bonus, and then complete the puzzle with at least 1 minute and 30 seconds remaining.
Clear a single-lap playlist consisting of the skins That Savanna Calls, Manager Class, Papa's Ukulele, The Bird Singing in the Night, and Song #4 in that order using only the bottom 4 rows.
Clear a single-lap playlist consisting of the skins So That Someone May Visit You, Water Flower & Lights, Fly into the Sky, Song #3, Day Dream, and Elect.M.G.R in that order always erasing at least 6 blocks per timeline pass.
Reach the skin Pump It on the second lap of Challenge B with a score of at least 1,300,000 having gotten at least 8 all-clear bonuses and erased at least 20 squares in a single timeline pass.
Clear a single-lap playlist consisting of the skins Fanatic, Whoop-De-Doo, Machine Interface, Xop, Tiny Piano, Go to a Toy Town, Flashback, 45 Degrees, and Song #1 in that order having gotten at least 105 single-color bonuses.
Clear a single-lap playlist consisting of the skins Star Guitar, Take My Time, Black Tambourine, Bottle Rocket, Pump It, Lose Control, Regret, and Heavenly Star in that order having erased at least 600 blocks using chain blocks.
Clear 2 full laps of an endless playlist consisting of the skins Naha!!, Cuckoo Clock, Butterfly Stroke, Song #2, The Speed of Light, The Mission to the Moon, Prime Factor, and Circles in that order only placing blocks in the middle 6 columns.
Complete a single-lap playlist consisting of the skins Tin Toy, Bebop Acid, Urbanization, Please Return my CD, 4x4 Bricks, Bloomy Girls, and El Viento in that order having erased at least 80 white squares more than non-white squares total.
Clear a single-lap playlist consisting of the skins Stroll Around the World, Aback, Talk 2 You, Holiday in Summer, Meguro, Spirits, Strangers, and Brash in that order with a score of at least 300,000 without rotating falling blocks more than once.