Complete Stage 1: Things Change with any turtle on normal or hard difficulty
Complete Stage 2: Nano with any turtle on normal or hard difficulty
Complete Stage 3: Trap with any turtle on normal or hard difficulty
Complete Stage 4: Tengu with any turtle on normal or hard difficulty
Complete Stage 5: Notes From the Underground with any turtle on normal or hard difficulty
Defeat Shredder in Stage 6: Justice as Raphael on normal or hard difficulty
Defeat Shredder in Stage 6: Justice as Michelangelo on normal or hard difficulty
Defeat Shredder in Stage 6: Justice as Donatello on normal or hard difficulty
Defeat Shredder in Stage 6: Justice as Leonardo on normal or hard difficulty
Defeat Oroku Saki in Stage 6: Justice with any turtle on normal or hard difficulty
Defeat Oroku Saki in Stage 6: Justice as Splinter on normal or hard difficulty
Defeat Oroku Saki in Stage 6: Justice as Casey Jones on normal or hard difficulty
Collect all 15 red Makimono scrolls
Collect all 15 orange Makimono scrolls
Collect all 15 purple Makimono scrolls
Collect all 15 blue Makimono scrolls
Collect all 32 gray Makimono scrolls
Unlock Casey Jones in Story Mode by completing any level after collecting all unlockables, abilities, and Makimono scrolls
In Versus Mode, defeat Michelangelo (CPU) without taking damage using Raphael at the Subway Station on hard
In Versus Mode, defeat Evil Turtlebot (CPU) without taking damage using Michelangelo in the Air on hard
In Versus Mode, defeat Leonardo (CPU) without taking damage using Donatello on the Rooftop on hard
In Versus Mode, defeat Shredder (CPU) without taking damage using Leonardo at the Heliport on hard
In Versus Mode, defeat Casey Jones (CPU) without taking damage using Hun on the Streets on hard
In Versus Mode, defeat Hun (CPU) without taking damage using Shredder at the Elevator Hall on hard
In Versus Mode, defeat Oroku Saki (CPU) without taking damage using Splinter at the Shinto Palace on hard
In Versus Mode, defeat Hamato Yoshi (CPU) without taking damage using Oroku Saki at Hamato Yoshi's Dojo on hard
In Versus Mode, defeat Splinter (CPU) without taking damage using Hamato Yoshi at Home on hard
In Versus Mode, defeat Donatello (CPU) without taking damage using Evil Turtlebot at the Junk Yard Rear on hard
In Versus Mode, defeat Raphael (CPU) without taking damage using Casey Jones on the Trailor Roof on hard
Complete Challenge Mode as Raphael on normal or hard difficulty
Complete Challenge Mode as Michelangelo on normal or hard difficulty
Complete Challenge Mode as Donatello on normal or hard difficulty
Complete Challenge Mode as Leonardo on normal or hard difficulty
Complete Challenge Mode using any turtle on hard difficulty
Perform a 20 hit combo in Story Mode
Perform a 50 hit combo in Story Mode
Complete Stage 1: Things Change with any turtle on normal or hard difficulty without using gembu, shuriken, or dying
Complete Stage 3: Trap with any turtle on normal or hard difficulty without using gembu, shuriken, or dying
Complete Stage 4: Tengu with any turtle on normal or hard difficulty without using gembu, shuriken, or dying
Complete Stage 6: Justice with any turtle on normal or hard difficulty without using gembu, shuriken, or dying (includes Oroku Saki battle in Area 8)
Complete Stage 2: Nano with any turtle on normal or hard difficulty without using gembu, shuriken, or dying
Complete Stage 5: Notes From the Underground with any turtle on normal or hard difficulty without using gembu, shuriken, or dying
Complete Stage 1: Things Change as any character with 325,000 or more points on any difficulty
Complete Stage 2: Nano as any character with 325,000 or more points on any difficulty
Complete Stage 3: Trap as any character with 400,000 or more points on any difficulty
Complete Stage 4: Tengu as any character with 300,000 or more points on any difficulty
Complete Stage 5: Notes From the Underground as any character with 375,000 or more points on any difficulty
Complete either Stage 6-7: Justice - Heliport or 6-8 - Shinto Palace as any character with 675,000 or more points on any difficulty
Complete Dojo: The Secrets as any character with 10,000 or more points on any difficulty
Complete Challenge Mode as any turtle with 450,000 or more points on any difficulty
Complete Dojo: The Secrets with any turtle on any difficulty within 30 seconds
Complete Dojo: Progress with Raphael on hard within 2:30
Complete Dojo: Progress with Michelangelo on any difficulty within 12 seconds
Complete Dojo: Progress with Donatello on hard within 30 seconds
Complete Dojo: Progress with Leonardo on hard within 1 minute
Complete Special Stage: Ordeal with Raphael, Michelangelo, or Donatello on hard within 20 seconds
Complete Special Stage: Ordeal with Leonardo on hard within 25 seconds
Complete Challenge Mode with any turtle on any difficulty within 15 minutes
Complete Stage 1: Things Change with any turtle on any difficulty within 12:30
Complete Stage 2: Nano with any turtle on any difficulty within 11:00
Complete Stage 3: Trap with any turtle on any difficulty within 13:30
Complete Stage 4: Tengu with any turtle on any difficulty within 12:30
Complete Stage 5: Notes From the Underground with any turtle on any difficulty within 12:30
Complete Stage 6: Justice with any turtle on any difficulty within 22:00 (Includes Oroku Saki fight in 6-8)
Complete Stage 6: Justice with any turtle on hard difficulty without using gembu (includes Oroku Saki battle in Area 8)