Complete Stage 1: Things Change with any turtle on normal or hard difficulty

Complete Stage 2: Nano with any turtle on normal or hard difficulty

Complete Stage 3: Trap with any turtle on normal or hard difficulty

Complete Stage 4: Tengu with any turtle on normal or hard difficulty

Complete Stage 5: Notes From the Underground with any turtle on normal or hard difficulty

Defeat Shredder in Stage 6: Justice as Raphael on normal or hard difficulty

Defeat Shredder in Stage 6: Justice as Michelangelo on normal or hard difficulty

Defeat Shredder in Stage 6: Justice as Donatello on normal or hard difficulty

Defeat Shredder in Stage 6: Justice as Leonardo on normal or hard difficulty

Defeat Oroku Saki in Stage 6: Justice with any turtle on normal or hard difficulty

Defeat Oroku Saki in Stage 6: Justice as Splinter on normal or hard difficulty

Defeat Oroku Saki in Stage 6: Justice as Casey Jones on normal or hard difficulty

Collect all 15 red Makimono scrolls

Collect all 15 orange Makimono scrolls

Collect all 15 purple Makimono scrolls

Collect all 15 blue Makimono scrolls

Collect all 32 gray Makimono scrolls

Unlock Casey Jones in Story Mode by completing any level or Challenge Mode after collecting all unlockables, abilities, and Makimono scrolls

In Versus Mode, defeat Michelangelo (CPU) without taking damage using Raphael at the Subway Station on hard

In Versus Mode, defeat Evil Turtlebot (CPU) without taking damage using Michelangelo in the Air on hard

In Versus Mode, defeat Leonardo (CPU) without taking damage using Donatello on the Rooftop on hard

In Versus Mode, defeat Shredder (CPU) without taking damage using Leonardo at the Heliport on hard

In Versus Mode, defeat Casey Jones (CPU) without taking damage using Hun on the Streets on hard

In Versus Mode, defeat Hun (CPU) without taking damage using Shredder at the Elevator Hall on hard

In Versus Mode, defeat Oroku Saki (CPU) without taking damage using Splinter at the Shinto Palace on hard

In Versus Mode, defeat Hamato Yoshi (CPU) without taking damage using Oroku Saki at Hamato Yoshi's Dojo on hard

In Versus Mode, defeat Splinter (CPU) without taking damage using Hamato Yoshi at Home on hard

In Versus Mode, defeat Donatello (CPU) without taking damage using Evil Turtlebot at the Junk Yard Rear on hard

In Versus Mode, defeat Raphael (CPU) without taking damage using Casey Jones on the Trailor Roof on hard

Complete Challenge Mode as Raphael on normal or hard difficulty

Complete Challenge Mode as Michelangelo on normal or hard difficulty

Complete Challenge Mode as Donatello on normal or hard difficulty

Complete Challenge Mode as Leonardo on normal or hard difficulty

Complete Challenge Mode using any turtle on hard difficulty

Perform a 20 hit combo in Story Mode

Perform a 50 hit combo in Story Mode

Complete Stage 1: Things Change with any turtle on normal or hard difficulty without using gembu, shuriken, or dying

Complete Stage 3: Trap with any turtle on normal or hard difficulty without using gembu, shuriken, or dying

Complete Stage 4: Tengu with any turtle on normal or hard difficulty without using gembu, shuriken, or dying

Complete Stage 6: Justice with any turtle on normal or hard difficulty without using gembu, shuriken, or dying (includes Oroku Saki battle in Area 8)

Complete Stage 2: Nano with any turtle on normal or hard difficulty without using gembu, shuriken, or dying

Complete Stage 5: Notes From the Underground with any turtle on normal or hard difficulty without using gembu, shuriken, or dying

Complete Stage 1: Things Change as any character with 325,000 or more points on any difficulty

Complete Stage 2: Nano as any character with 325,000 or more points on any difficulty

Complete Stage 3: Trap as any character with 400,000 or more points on any difficulty

Complete Stage 4: Tengu as any character with 300,000 or more points on any difficulty

Complete Stage 5: Notes From the Underground as any character with 375,000 or more points on any difficulty

Complete either Stage 6-7: Justice - Heliport or 6-8 - Shinto Palace as any character with 675,000 or more points on any difficulty

Complete Dojo: The Secrets as any character with 10,000 or more points on any difficulty

Complete Challenge Mode as any turtle with 450,000 or more points on any difficulty

Complete Dojo: The Secrets with any turtle on any difficulty within 30 seconds

Complete Dojo: Progress with Raphael on hard within 2:30

Complete Dojo: Progress with Michelangelo on any difficulty within 12 seconds

Complete Dojo: Progress with Donatello on hard within 30 seconds

Complete Dojo: Progress with Leonardo on hard within 1 minute

Complete Special Stage: Ordeal with Raphael, Michelangelo, or Donatello on hard within 20 seconds

Complete Special Stage: Ordeal with Leonardo on hard within 25 seconds

Complete Challenge Mode with any turtle on any difficulty within 15 minutes

Complete Stage 1: Things Change with any turtle on any difficulty within 12:30

Complete Stage 2: Nano with any turtle on any difficulty within 11:00

Complete Stage 3: Trap with any turtle on any difficulty within 13:30

Complete Stage 4: Tengu with any turtle on any difficulty within 12:30

Complete Stage 5: Notes From the Underground with any turtle on any difficulty within 12:30

Complete Stage 6: Justice with any turtle on any difficulty within 22:00 (Includes Oroku Saki fight in 6-8)

Complete Stage 6: Justice with any turtle on hard difficulty without using gembu (includes Oroku Saki battle in Area 8)